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empty pspliceexpress vector as a negative control  (Amaxa)

 
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    Amaxa empty pspliceexpress vector as a negative control
    Empty Pspliceexpress Vector As A Negative Control, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+pspliceexpress+vector+as+a+negative+control/empty+pspliceexpress+vector+as+a+negative+control/10__1590_slash_1678___4685___gmb___2016___0234-66-26-35
    Average 90 stars, based on 1 article reviews
    empty pspliceexpress vector as a negative control - by Bioz Stars, 2026-10
    90/100 stars

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    Transfection:

    Article Title: Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models
    Article Snippet: .. Then the cells (2 x 106) were transfected with methylated and unmethylated pSpliceExpress cassettes containing the CpG island DNA element (2 g), together with the empty pSpliceExpress vector as a negative control, by nucleofection using Amaxa® Cell Line Nucleofector® Kit V (Amaxa, Cologne, Germany). .. Transfected cells were incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2, and RNA isolation was performed using High Pure RNA Isolation Kit (Roche Diagnostics, Mannheim, Germany). cDNA synthesis was done using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Inc. Foster City, California).

    Methylation:

    Article Title: Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models
    Article Snippet: .. Then the cells (2 x 106) were transfected with methylated and unmethylated pSpliceExpress cassettes containing the CpG island DNA element (2 g), together with the empty pSpliceExpress vector as a negative control, by nucleofection using Amaxa® Cell Line Nucleofector® Kit V (Amaxa, Cologne, Germany). .. Transfected cells were incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2, and RNA isolation was performed using High Pure RNA Isolation Kit (Roche Diagnostics, Mannheim, Germany). cDNA synthesis was done using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Inc. Foster City, California).

    Negative Control:

    Article Title: Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models
    Article Snippet: .. Then the cells (2 x 106) were transfected with methylated and unmethylated pSpliceExpress cassettes containing the CpG island DNA element (2 g), together with the empty pSpliceExpress vector as a negative control, by nucleofection using Amaxa® Cell Line Nucleofector® Kit V (Amaxa, Cologne, Germany). .. Transfected cells were incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2, and RNA isolation was performed using High Pure RNA Isolation Kit (Roche Diagnostics, Mannheim, Germany). cDNA synthesis was done using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Inc. Foster City, California).



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    empty pspliceexpress vector as a negative control  (Amaxa)
    90
    Amaxa empty pspliceexpress vector as a negative control
    Empty Pspliceexpress Vector As A Negative Control, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+pspliceexpress+vector+as+a+negative+control/empty+pspliceexpress+vector+as+a+negative+control/10__1590_slash_1678___4685___gmb___2016___0234-66-26-35
    Average 90 stars, based on 1 article reviews
    empty pspliceexpress vector as a negative control - by Bioz Stars, 2026-10
    90/100 stars
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